Scientist, Proteomics-----Cambridge, MA

Key Responsibilities: Design and execute discovery proteomic assaysusingmass spectrometryto support mechanistic studies, Client Client targets, and elucidate biological regulators of disease biology. Performproximity biotinylation studies in cells, using establishedautomated affinity purificationworkflows, and data independent acquisition(DIA) mass spectrometrymethods. Performtarget validation by developing proximity ligation assays (PLA) and cell-based assaysto assess protein-protein interactions using chemical tools and confocal microscopy. Generateprotein conjugates and characterize protein labeling by UV-Vis spectroscopy. Analyze and interpret experimental data using established bioinformatic pipelines. Maintain detailed and accurateelectronic laboratory notebooks. Represent CBP team, and communicate with project discipline leads, and contribute to generation of mechanistic hypothesis through interpretation of proteomics data. Ph.D. in Proteomics, Chemical Biology, Biological Sciences, Biochemistry, or a related discipline, with at l east 2 years of hands-on proteomics experience, including experience gained during doctoral or postdoctoral research. OrMasters degree with 3-5 years of hands-on proteomics experience. Knowledge and hands-on experience withproteomic sample preparation. Experience inmammalian cell culture .Experience employing cell-based and proteomic assays to understandsmall molecule mechanisms of action. Demonstrated ability to work effectively both independently and collaboratively, contribute to scientific discussions, solve problems, and deliver high-quality results within agreed timelines.

Preferred Background: Experience developing or applying proximity ligation assays or related proximity-basedprotein-protein interaction validation assays. Experience using LC-MS/MS platformsand semi-automated instrumentation. Experience usingproteomics search and analysis tools, such as Spectronaut, Proteome Discoverer, MaxQuant, Skyline and Xcalibur.